Protein Modification Screencast
In this tutorial I would like to show you some basics of a protein modification and corresponding data interpretation using mMass. The data I am using in this video corresponds to Aldolase A from rabbit modified by p-chloromercury benzoate and digested by trypsin. Mass spectrum was acquired on a MALDI-TOF mass spectrometer.
In this video you will learn how to perform the following tasks:
- how to define new modification,
- how to get a protein sequence from UniProt,
- how to apply fixed or variable modifications,
- how to perform in-silico digestion,
- how to match theoretical data to acquired spectrum,
- how to annotate matched peptides,
- how to generate and save theoretical isotopic pattern,
- how to generate Analysis Report.
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Watch this video on YouTube.