Protein Identification Screencast
In this tutorial I would like to show you some basics of a protein identifications using mMass. The data I am using in this video corresponds to an in-gel digestion of a single band from 1-D electrophoresis. The sample was reduced by TCEP, alkylated by Iodoacetamide and digested by Trypsin. Mass spectrum was measured on a high-resolution FT-ICR mass spectrometer using MALDI ionization technique.
In this video you will learn how to perform the following tasks:
- how to label peaks,
- how to send data to Mascot's PMF tool,
- how to define a new protein sequence and apply modifications,
- how to perform in-silico digestion,
- how to match theoretical data to acquired spectrum,
- how to annotate matched peptides,
- how to search unmatched peaks by Mascot's PMF tool,
- how to search for non-specific peptides,
- how to generate theoretical isotopic pattern,
- how to generate Analysis Report.
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Watch this video on YouTube.